Gene Expression Profiling of the Sciatic Nerve in Streptozotocin-Induced Diabetic Rats with Peripheral Neuropathy
Table 1
Primers used in qRT-PCR validation.
Gene
Primer sequences
Products’ length
(°C)
Mmp9
F: 5-ACCCTGCGTATTTCCATTCATC-3
169
60
R: 3-CGAGTTGCCCCCAGTTACAGT-5
60
Mapk12
F: 5-CTTGAGGAATGGAAGCGTGTT-3
274
60
R: 3-TAGTGCTTGGGAGGTAGAGG-5
60
Agt
F: 5-GGTTCTCAACAGCATCCTCCTT-3
145
60
R: 3-ACCTGAGTCCCGCTCGTAGA-5
60
Adipoq
F: 5-TGTTCCTCTTAATCCTGCCCAG-3
239
60
R: 3-TCCTGTCATTCCAGCATCTCCT-5
60
Gapdh
F: 5-TTCCTACCCCCAATGTATCCG-3
281
60
R: 3-CATGAGGTCCACCACCCTGTT-5
60
Four genes including 2 upregulated genes (Mmp9 and Mapk12) and 2 downregulated genes (Agt and Adipoq) were selected for qRT-PCR validation. Gapdh was used as the endogenous reference gene.