Gene Expression Profiling of the Sciatic Nerve in Streptozotocin-Induced Diabetic Rats with Peripheral Neuropathy

Table 1

Primers used in qRT-PCR validation.

Gene	Primer sequences	Products’ length	(°C)
Mmp9	F: 5-ACCCTGCGTATTTCCATTCATC-3	169	60
	R: 3-CGAGTTGCCCCCAGTTACAGT-5		60
Mapk12	F: 5-CTTGAGGAATGGAAGCGTGTT-3	274	60
	R: 3-TAGTGCTTGGGAGGTAGAGG-5		60
Agt	F: 5-GGTTCTCAACAGCATCCTCCTT-3	145	60
	R: 3-ACCTGAGTCCCGCTCGTAGA-5		60
Adipoq	F: 5-TGTTCCTCTTAATCCTGCCCAG-3	239	60
	R: 3-TCCTGTCATTCCAGCATCTCCT-5		60
Gapdh	F: 5-TTCCTACCCCCAATGTATCCG-3	281	60
	R: 3-CATGAGGTCCACCACCCTGTT-5		60

Four genes including 2 upregulated genes (Mmp9 and Mapk12) and 2 downregulated genes (Agt and Adipoq) were selected for qRT-PCR validation. Gapdh was used as the endogenous reference gene.