Change in Viability and Function of Pancreatic Islets after Coculture with Mesenchymal Stromal Cells: A Systemic Review and Meta-Analysis

Table 1

Characteristics of the eligible studies included in the meta-analysis.

Author [reference]	Year	Outcome evaluated	Technique used	Viability test	Islet origin/MSC tissue origin	Type of coculture	Time of coculture
Arzouni et al. [18]	2017	ISI	Radioimmunoassay	—	Human/hAD-MSC	Direct	4 d
Arzouni et al. [19]	2019	ISI	Radioimmunoassay	—	Human, mouse/hAD-MSC, hBM-MSC, mAD-MSC, mKidney-MSC	Direct	1 d/2 d/3 d
Chen et al. [38]	2013	Viability, ISI	Radioimmunoassay	AO/PI	Rat/rBM-MSC	Direct	2 w
Davis et al. [27]	2012	ISI	ELISA	—	Mouse/mBM-MSC	Encapsulation	7 d
Duprez et al. [12]	2011	ISI	ELISA	—	Human/hBM-MSC	Direct	2 d
Jun et al. [28]	2014	ISI, viability	ELISA	Calcein AM/ethidium homodimer-1	Rat/rAD-MSC	Encapsulation	7 d/14 d
Jung et al. [13]	2011	Viability, VEGF, TNF-α, ISI	ELISA	FDA/PI	Rat/rBM-MSC	Indirect/direct	1 w/4 w
Karaoz et al. [29]	2010	Viability, IL-6, ISI	ELISA	FDA/PI	Rat/rBM-MSC	Indirect	2 w
Karaoz et al. [11]	2011	Insulin	ELISA		Rat/rBM-MSC	Direct	16 d
Kerby et al. [30]	2013	ISI	Radioimmunoassay		Mouse/mKidney-MSC	Encapsulation	3 d
Kono et al. [31]	2014	Viability, VEGF	—	EH 1/calcein AM	Mouse/hAD-MSC	Indirect	6 d
Lu et al. [33]	2010	ISI	ELISA	—	Rat/rBM-MSC	Direct	3 d/4 d
Ayenehdeh et al. [20]	2017	Insulin	ELISA	—	Mouse/mAD-MSC	Encapsulation	—
Montanari et al. [15]	2017	ISI	ELISA	—	Human/hBM-MSC	Indirect/direct	3 d
Park et al. [25]	2009	ISI, VEGF, IL-6, TNF-α	ELISA	—	Human/hBM-MSC, hUCB-MSC	Indirect	2 d
Park et al. [26]	2010	ISI, viability	ELISA	AO/PI	Mouse/hUCB-MSC	Indirect	2 d
Rackham et al. [14]	2013	ISI	Radioimmunoassay	—	Mouse/mKidney-MSC	Indirect/direct	3 d
Rackham et al. [34]	2014	ISI	Radioimmunoassay	—	Mouse/mAD-MSC	Direct	3 d
Rackham et al. [35]	2016	ISI	Radioimmunoassay		Mouse/mAD-MSC	Direct	3 d
Scuteri et al. [16]	2014	Viability, insulin	ELISA	Calcein AM	Rat/rBM-MSC	Indirect/direct	1 w
Yamada et al. [36]	2014	Viability, IL-6, VEGF, TNF-α, insulin	ELISA	Trypan blue staining	Pig/hAD-MSC	Indirect	2 d
Yoshimatsu et al. [37]	2013	Viability, ISI	ELISA	Syto-Green/EB	Rat/rBM-MSC	Direct/encapsulation	3 d
Yoshimatsu et al. [9]	2015	Viability, ISI, insulin	ELISA	Syto-Green/EB	Mouse/mBM-MSC	Direct	1 d/4 d
Ren et al. [21]	2019	—	—	—	Mouse/mAD-MSC	Cotransplantation	—

Note. MSC = mesenchymal stromal cells; ISI = insulin stimulation index; FDA/PI = fluorescein diacetate/propidium iodide; EB = ethidium bromide; EH 1 = ethidium homodimer-1; VEGF = vascular endothelial growth factor; IL-6 = interleukin-6; TNF-α = tumor necrosis factor-α; h = human; m = mouse; BM = bone marrow; UCB = umbilical cord blood; AD = adipose; d = day; w = week.