Green Coffea arabica Extract Ameliorates Testicular Injury in High-Fat Diet/Streptozotocin-Induced Diabetes in Rats

Table 1

Primer sequences of genes analyzed in real-time PCR.

Name	Accession number	Sense (5—3)	Antisense (5—3)
Actb	NM_031144.3	GCAGGAGTACGATGAGTCCG	ACGCAGCTCAGTAACAGTCC
Sod2	NM_001270850.1	AGCTGCACCACAGCAAGCAC	TCCACCACCCTTAGGGCTCA
Cat	NM_012520.2	TCCGGGATCTTTTTAACGCCATTG	TCGAGCACGGTAGGGACAGTTCAC
Gpx1	NM_017006.2	CGGTTTCCCGTGCAATCAGT	ACACCGGGGACCAAATGATG
Gsr	NM_053906.2	TGCACTTCCCGGTAGGAAAC	GATCGCAACTGGGGTGAGAA
Bcl2	NM_016993.1	CTGGTGGACAACATCGCTCTG	GGTCTGCTGACCTCACTTGTG
Bax	NM_017059.2	GGCGAATTGGCGATGAACTG	ATGGTTCTGATCAGCTCGGG
Tnfα	XM_008772775.2	AGAACTCAGCGAGGACACCAA	GCTTGGTGGTTTGCTACGAC
Il1β	NM_031512.2	GACTTCACCATGGAACCCGT	GGAGACTGCCCATTCTCGAC
Nos2	NM_012611.3	GTTCCTCAGGCTTGGGTCTT	TGGGGGAACACAGTAATGGC

The abbreviations of the genes: Actb: β-actin; Sod2: manganese-dependent superoxide dismutase (MnSOD); Cat: catalase; Gpx1: glutathione peroxidase; Gsr: glutathione reductase; Bcl2: B-cell lymphoma 2; Bax: Bcl-2-like protein 4; Tnfα: tumor necrosis factor alpha; Il1β: interleukin-1 beta; Nos2: nitric oxide synthase 2.