miR-210 in Exosomes Derived from Macrophages under High Glucose Promotes Mouse Diabetic Obesity Pathogenesis by Suppressing NDUFA4 Expression

Figure 1

Extraction and characterization of exosomes from mouse macrophage under high glucose treatment. (a, b) Protein abundances of HSP70, CD63, and TSG101 in exosomes extracted from Raw264.7 cells treated with high glucose. Protein abundances were determined by western blotting (a) and quantitated for statistical analysis (b). The remaining supernatant of Raw264.7 cell culture following exosome extraction was used as the control group. GAPDH was used as the internal standard. (c) Observation of exosomes from mouse macrophage under high glucose treatment by electron microscopy. . (d) miR-210 levels in exosomes derived from Raw264.7 cells treated with high glucose. The miR-210 levels were measured by quantitative RT-PCR levels. NG-exo: normal glucose exosomes; HG-exo: high glucose exosomes; HSP70: heat shock protein 70; TSG101: tumor susceptibility gene 101; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; and (compared with the control group); ^## (compared with the normal glucose exosome group).

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