miR-210 in Exosomes Derived from Macrophages under High Glucose Promotes Mouse Diabetic Obesity Pathogenesis by Suppressing NDUFA4 Expression

Figure 4

NDUFA4 overexpression counteracted the inhibition of adipocyte glucose uptake and CIV activity by exosomes derived from macrophages under high glucose. (a) Glucose uptake in NDUFA4-overexpressing 3T3-L1 cells treated with exosomes from RAW-264.7 cells under high glucose treatment. Glucose levels in 3T3-L1 cells were measured by the fluorometric method. (b) CIV activity in NDUFA4-overexpressing 3T3-L1 cells treated with exosomes derived from RAW-264.7 cells under high glucose treatment. (c) NDUFA4 mRNA levels in NDUFA4-overexpressing 3T3-L1 cells treated with exosomes extracted from RAW-264.7 cells under high glucose. The quantitative RT-PCR method was used to analyze NDUFA4 mRNA levels. (d) NDUFA4 protein levels in NDUFA4-overexpressing 3T3-L1 cells treated with exosomes derived from high glucose-induced RAW-264.7 cells. VDAC1 was used as the internal standard. NDUFA4: NADH dehydrogenase (ubiquinone) 1 alpha subcomplex 4; HG: high glucose; exo: exosomes; CIV: complex IV; ; .

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