miR-210 in Exosomes Derived from Macrophages under High Glucose Promotes Mouse Diabetic Obesity Pathogenesis by Suppressing NDUFA4 Expression

Figure 5

miR-210 knockout suppressed diabetic obesity progression and elevated NDUFA4 expression in a mouse model. (a) The level of miR-210 was determined by RT-PCR analysis in exosome derived from serum of miR-210 WT and KO diabetic obesity model mice. (b) Blood glucose content in the diabetic obesity model established using the miR-210 knockout mice. Glucose content in the mouse blood was analyzed by the fluorometric method. (c, d) Body weight (c) and the brown adipose tissue weight (d) were detected on the diabetic obesity model established using the miR-210 knockout mice. (e) NDUFA4 mRNA levels in the diabetic obesity model established using the miR-210 knockout mice. Quantitative RT-PCR was performed to analyze the NDUFA4 mRNA levels. (f) NDUFA4 protein abundances in the diabetic obesity model established using the miR-210 knockout mice by western blotting. VDAC1 was used as the internal standard. NDUFA4: NADH dehydrogenase (ubiquinone) 1 alpha subcomplex 4; VDAC1: voltage-dependent anion-selective channel 1; WT: wild type; KO: knockout; OD: obese diabetes. and (compared with the WT group); (compared with the WT+OD group).

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