Regulation and Mechanism of miR-518d through the PPARα-Mediated NF-κB Pathway in the Development of Gestational Diabetes Mellitus

Figure 5

Regulation of miR-518d through the PPARα-mediated NF-κB pathway in GDM mice. (a) Body weights of mice in both Control_Ms and GDM_Ms groups recorded on the 0, 3, 10, and 18 days after pregnancy. (b) IPGTT on the 18^th day of pregnancy after 16-hour fasting. (c) Pathological indicator (CH, TG, FBG, and INS) tests on the 18^th day of pregnancy after 12-hour fasting. (d) HOMA-IR calculated based on the data obtained from pathological tests. (e) Expression of miR-518d after transfection. Expression levels of mRNA and proteins of PPARα and its downstream genes detected by (f) RT-PCT and (g) Western blotting, respectively. (h) Contents of relative inflammatory factors (NF-κB, TNF-α, IL-1β, IL-6, and COX-2) in peripheral plasma examined by ELISA. (i) Relative expressions in the nucleus of NF-κB and pathway-related proteins detected by Western blotting. All data were represented as . , .

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