Geranylgeranyl Transferase-I Knockout Inhibits Oxidative Injury of Vascular Smooth Muscle Cells and Attenuates Diabetes-Accelerated Atherosclerosis
Conditional knockout of VSMC GGTase-I inhibits the activation of small GTPase, Rac1, both in vivo and in vitro. (a) Rac1 activities were determined in aortas from four groups. (b) VSMCs were isolated from wild-type and Pggt1bΔ/Δ mice and treated with normal glucose (5.6 mM) or high glucose (22.2 mM) for 72 h. VSMCs from wild-type mice were also cultured with selective GGTase-I inhibitor (GGTI-286, 10 μM) or Rac1 inhibitor (100 μM) for 24 h. Rac1 activities were measured by the Rac1 G-LISA Activation Assay Kit according to the manufacturer’s instruction. Data are expressed as , for each group. versus strain-matched nondiabetic mice (or strain-matched normal glucose VSMCs). # and ## versus diabetic wild-type mice (or wild-type high-glucose VSMCs).
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