Geranylgeranyl Transferase-I Knockout Inhibits Oxidative Injury of Vascular Smooth Muscle Cells and Attenuates Diabetes-Accelerated Atherosclerosis

Figure 6

Effects of GGTase-I knockout on p47phox and NOXO1 in cytosolic and membrane fractions from cultured VSMCs in vitro and aortic tissue in vivo. The protein expressions were detected by western blot analysis. (a) Representative blots and (c) densitometric average in membrane fractions from aortic tissue in vivo. (b) Representative blots and (d) densitometric average in cytosolic fractions from aortic tissue in vivo. (e) Representative blots and (f, g) densitometric average in membrane fractions from cultured VSMCs in vitro. VSMCs were isolated from wild-type and Pggt1b^Δ/Δ mice and treated with normal glucose (5.6 mM) or high glucose (22.2 mM) for 72 h. VSMCs from wild-type mice were also cultured with a selective GGTase-I inhibitor (GGTI-286, 10 μM) or Rac1 inhibitor (100 μM) for 24 h. β-Actin and Na⁺/K⁺-ATPase were used as loading controls for the cytoplasm and plasma membrane, respectively. Data are expressed as , for each group. and versus strain-matched nondiabetic mice (or strain-matched normal glucose VSMCs). ^## versus diabetic wild-type mice (or wild-type high-glucose VSMCs).

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