Geranylgeranyl Transferase-I Knockout Inhibits Oxidative Injury of Vascular Smooth Muscle Cells and Attenuates Diabetes-Accelerated Atherosclerosis
Effects of GGTase-I knockout on the phosphorylation of ERK1/2, JNK, and p38 in cultured VSMCs in vitro and aortic tissue in vivo. Western blot analysis of ERK1/2 (a), JNK (b), and p38 (c) phosphorylation and total protein expression in aortic tissue from four groups. Western blot of ERK1/2 (d), JNK (e), and p38 (f) phosphorylation and total protein expression in cultured VSMCs in vitro. VSMCs were isolated from wild-type and Pggt1bΔ/Δ mice and treated with normal glucose (5.6 mM) or high glucose (22.2 mM) for 72 h. VSMCs from wild-type mice were also cultured with a selective GGTase-I inhibitor (GGTI-286, 10 μM) or Rac1 inhibitor (100 μM) for 24 h. β-Actin was used as an internal control. Data expressed as , for each group. and versus strain-matched nondiabetic mice (or strain-matched normal glucose VSMCs). # and ## versus diabetic wild-type mice (or wild-type high-glucose VSMCs).
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