Tangeretin Inhibition of High-Glucose-Induced IL-1β, IL-6, TGF-β1, and VEGF Expression in Human RPE Cells

Figure 4

Tangeretin inhibited the mRNA expression of IL-1β, IL-6, TGF-β1, and VEGF in RPE cells. RPE cells were pretreated with various concentrations of tangeretin (5 μM, 10 μM, and 20 μM) for 1 h and then stimulated by the addition of HG (30 mM) for 24 h. A real-time PCR analysis was performed to assess the expression of IL-1β, IL-6, TGF-β1, and VEGF. Tangeretin significantly decreased the HG-induced mRNA (a–d) level of IL-1β, IL-6, TGF-β1, and VEGF in RPE cells in a dose-dependent manner. Values are expressed as the of three independent experiments. versus NG; versus NG; ^# versus HG.

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