Tangeretin Inhibition of High-Glucose-Induced IL-1β, IL-6, TGF-β1, and VEGF Expression in Human RPE Cells

Figure 5

Tangeretin decreased the protein level of IL-1β, IL-6, TGF-β1, and VEGF in RPE cells. After pretreatment with various concentrations of tangeretin (5 μM, 10 μM, and 20 μM) for 1 h, RPE cells were stimulated by the addition of HG (30 mM) for 24 h. An ELISA kit analysis was performed to assess the protein level of IL-1β, IL-6, TGF-β1, and VEGF. Tangeretin significantly decreased the HG-induced protein (a–d) levels of IL-1β, IL-6, TGF-β1, and VEGF in RPE cells in a dose-dependent manner. Values are expressed as the of three independent experiments. versus NG; ^# versus HG.

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