Tangeretin Inhibition of High-Glucose-Induced IL-1β, IL-6, TGF-β1, and VEGF Expression in Human RPE Cells

Figure 6

Inhibition of Akt signalling downregulated HG-induced IL-1β, IL-6, TGF-β1, and VEGF mRNA expression in RPE cells. RPE cells were preincubated for 1 h with 10 μM of LY294002 and then stimulated by HG (30 mM) for 24 h. The IL-1β, IL-6, TGF-β1, and VEGF mRNA levels were determined using real-time PCR. LY294002 significantly decreased the HG-induced mRNA (a–d) levels of IL-1β, IL-6, TGF-β1, and VEGF in RPE cells. The data shown represent the of three independent experiments. versus NG; ^# versus HG; ^## versus HG.

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