Liraglutide Improves Endothelial Function via the mTOR Signaling Pathway

Figure 2

HUVECs were administered liraglutide at 100 nM for 60 min. Immunoprecipitation (IP) was performed with anti-mTOR antibodies; Western blot (WB) with anti-Rictor and anti-Raptor antibodies was next carried out to assess immunoprecipitated complexes (a). HUVECs were administered liraglutide at 100 nM for 60 min in three groups, with a vehicle control group assessed in parallel. The vehicle group was transfected with control siRNA, while HUVECs treated with liraglutide were transfected with control siRNA, Rictor-specific siRNAs, and Raptor-specific siRNAs, respectively. Akt phosphorylation at Ser473 and total Akt levels were detected by Western blot (b). Akt phosphorylation levels relative to total Akt protein amounts were assessed by densitometric quantification. Data are (). vs. HUVECs transfected with control siRNA and treated with liraglutide (c).

(a)

(b)

(c)