Liraglutide Improves Endothelial Function via the mTOR Signaling Pathway

Figure 3

HUVECs were administered liraglutide at 100 nM in the absence or presence of Akt inhibitor IV for 60 min. NO release from HUVECs was assayed by the nitrate reductase method. Data are (). vs. the control group (a). Phosphorylated eNOS was detected by Western blot (b). Immunoprecipitation (IP) was performed with anti-eNOS antibodies, followed by Western blot (WB) with anti-hsp90 antibodies for assessing immunoprecipitated complexes (c).

(a)

(b)

(c)