Hyperinsulinemia Induced Altered Insulin Signaling Pathway in Muscle of High Fat- and Carbohydrate-Fed Rats: Effect of Exercise

Table 2

Effect of diet-induced hyperinsulinemia and exercise on lactate dehydrogenase activity and total lactate levels.

	LDH activity; (μmol min^-1/μg protein)	Total lactate levels; (mmol/l)
Control
HFD	^†	^††
HFD Ex	^††
HCD	^†	^††
HCD Ex	^††

Kinetic activity of LDH enzyme and lactate concentrations in rat muscle homogenate. The rate of oxidation of NADH which is directly proportional to LDH activity was read at the end of 10 minutes among the groups control, high-fat diet (HFD), high-fat diet exercise (HFD Ex), high-carbohydrate diet (HCD), and high-carbohydrate diet exercise (HCD Ex) at 340 nm. Lactate levels determined according to lactate assay kit protocol. Values are of 4–6 separate experiments ( animals per group). ^† and ^†† when compared to control, when compared to HFD and HCD.