HDL Improves Cholesterol and Glucose Homeostasis and Reduces Atherosclerosis in Diabetes-Associated Atherosclerosis

<div>rHDL alters expression of genes regulating cholesterol metabolism and inflammation. <i>Trail<sup>-/-</sup>Apoe<sup>-/-</sup></i> and <i>Apoe<sup>-/-</sup></i> mice were fed a high-cholesterol diet for 12 weeks. In the last 2 weeks of the study, mice received 3 weekly infusions of either rHDL (20 mg/kg) or PBS. (a) <i>Abca1</i>, (b) <i>Abcg1</i>, (c) <i>Srb1</i>, (d) <i>Cd68</i>, (e) <i>F4/80</i>, and (f) <i>Cd206</i> mRNA expression in aortae. Aortae were isolated and mRNA extracted as described in Methods. mRNA expression was normalised to 18S (<span class="nowrap"><svg height="8.55521pt" id="M38" style="vertical-align:-0.2063904pt" version="1.1" viewbox="-0.0498162 -8.34882 27.8368 8.55521" width="27.8368pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.013,0,0,-0.013,0,0)"><path d="M495 86L479 114C446 82 419 66 409 66C401 66 401 72 406 97C420 166 436 231 453 297C489 435 454 448 428 448C406 448 384 439 354 422C305 394 222 327 161 247H159L183 345C200 415 194 448 173 448C143 448 82 410 23 351L38 325C64 349 95 371 105 371C111 371 116 365 109 336L25 -4L31 -12C50 -4 77 3 107 9C119 69 132 122 145 168C197 254 321 381 370 381C387 381 393 374 378 305L329 95C309 17 320 -12 345 -12C372 -12 430 19 495 86Z"></path></g><g transform="matrix(.013,0,0,-0.013,10.158,0)"><path d="M535 323V373H52V323H535ZM535 138V188H52V138H535Z"></path></g><g transform="matrix(.013,0,0,-0.013,21.421,0)"><path d="M456 178V225H360V632H320C217 496 115 347 20 206V178H280V106C280 40 276 34 189 27V0H445V27C364 34 360 39 360 106V178H456ZM280 225H82C149 335 214 431 278 520H280V225Z"></path></g></svg>-</span>6/group). 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330 22 240 22C152 22 124 173 124 313S148 602 238 602Z"></path></g><g transform="matrix(.013,0,0,-0.013,49.709,0)"><path d="M384 0V27C293 34 287 42 287 114V635C232 613 172 594 109 583V559L157 557C201 555 205 550 205 499V114C205 42 199 34 109 27V0H384Z"></path></g></svg>,</span> ANOVA used in all conditions. rHDL: reconstituted high-density lipoprotein; Apoe: apolipoprotein E; TRAIL: TNF-related apoptosis-inducing ligand.</div>

Journal of Diabetes Research

fig4

Figure 4

Figure 4: HDL Improves Cholesterol and Glucose Homeostasis and Reduces Atherosclerosis in Diabetes-Associated Atherosclerosis 