Experimental Study on the Effect of Allogeneic Endothelial Progenitor Cells on Wound Healing in Diabetic Mice

Figure 3

Phenotypic characterization of DB/DB EPCs and DB/+ EPCs. (a) DB/DB EPCs and DB/+ EPCs were cultured, and cells grew uniformly with cobblestone-like appearance. (b) Double-staining identification of EPCs. Dil-Ac-LDL-labeled cells showed red fluorescence, FITC-UEA-1-labeled cells showed green fluorescence, and the double-stained EPCs appeared yellow. The percentages of double-positive FITC-UEA-1/Dil-Ac-LDL cells were DB/+(92.23%) and DB/DB (89.72%). (c) Analyses of DB/DB EPC and DB/+ EPC surface markers CD34, CD133, and CD309 by flow cytometry. Three independent experiments were performed.

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