Ghrelin Mitigates High-Glucose-Induced Oxidative Damage and Apoptosis in Lens Epithelial Cells

Figure 3

Ghrelin inhibited HG-induced HLE cell apoptosis. HLE cells were incubated with ghrelin (0.1 μM) for 2 h and then exposed to HG for 24 h. (a) The number of trypan-positive cells was quantified, and at least 100 cells per dish were counted. (b) Quantitative analyses of the apoptosis rate in HLE cells. (c) AO/EB double stain of HLE cells after a treatment with ghrelin. (d) Quantitative analyses of the apoptosis rate in HLE cells. (e) Flow cytometric analysis was used to detect apoptosis rate. (f) Quantitative analyses of the apoptosis rate. (g) Ultrastructural changes in HLE cells induced by HG using transmission electron microscopy. (h) The expressions of Bax and Bcl-2 were detected by Western blot. (i) Quantitative analysis of Bax expression intensity relative to actin. (j) Quantitative analysis of Bcl-2 expression intensity relative to actin. vs. control group; ^# vs. HG group.

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