AGEs/RAGE Promote Osteogenic Differentiation in Rat Bone Marrow-Derived Endothelial Progenitor Cells via MAPK Signaling

Figure 3

Immunostaining for OCN and CD34 in the control group (untreated cells) and AGE- and β-glycerophosphate-induced EPCs. OCN (green) and CD34 (red) protein levels were notable in EPCs exposed to AGE for 7 days. Nuclei were stained with DAPI (blue). Merged images show colocalization of staining for OCN and CD34. Magnification, ×400. AGE: advanced glycation end product; EPC: endothelial progenitor cell; OCN: osteocalcin.