AGEs/RAGE Promote Osteogenic Differentiation in Rat Bone Marrow-Derived Endothelial Progenitor Cells via MAPK Signaling

Figure 7

MAPK pathway inhibitors PD98059, SB203580, and SP600125 prevent AGE-induced activation phosphorylated ERK protein, p38MAPK, and JNK in EPCs, respectively. EPCs were pretreated with PD98059 (50 μmol/l), SB203580 (20 μmol/l), or SP600125 (50 μmol/l) for 1 h, then stimulated with AGE (100 μg/ml) for 5 min. Untreated and DMSO-treated samples were used as the controls. (a) Total protein and phosphorylated ERK, p38, and JNK levels and GAPDH were measured by western blot. (b) Phosphorylation-to-total protein expression ratios of ERK, p38, and JNK are presented. Each data point represents the results of three independent experiments. Comparison with control, ^▲ vs.; comparison with DMSO, ^◆ vs. AGE: advanced glycation end product; Con: control; EPC: endothelial progenitor cell; p-: phosphorylated.

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