Enhancement of Mitochondrial Function by the Neurogenic Molecule NSI-189 Accompanies Reversal of Peripheral Neuropathy and Memory Impairment in a Rat Model of Type 2 Diabetes

Figure 6

NSI treatment restores mitochondrial complex I and cytochrome c oxidase activity in the brain cortex from ZDF rats in parallel with elevation of expression of respiratory chain proteins. Brain cortex tissues were obtained from control (Ctrl), ZDF, and NSI-treated ZDF (ZDF+NSI) rats and underwent (a–f) Western blotting or (g, h) complex I and complex IV activity assays. (b) Protein levels for (a) Cox I (NDUFB8), (b) Cox II (SDHB), (c) Cox III (UQCRC2), (d) Cox IV (MTCO1), and (e) Cox V (ATP5a). (f) Western blot image of subunits of mitochondrial complexes I-V and total ERK (T-ERK). (g) Complex I activity and (h) cytochrome c oxidase (Cox IV) activity. Data are of ; , , and , analyzed by one-way ANOVA with Holm-Sidak’s post hoc test.

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