Epigenetic Changes Induced by High Glucose in Human Pancreatic Beta Cells

Figure 3

Heat map of hypermethylated and hypomethylated gene promoters. MeDIP-Chip analysis grouped into clusters shows the heat map of differentially methylated gene promoters between glucose-treated (g1, g2, and g3) and control-treated (C1, C2, and C3) samples. The scale symbolizes hypomethylated promoters (values 0 to +2) in green and hypermethylated promoters (values 0 to −2) in red. Each tiny row represents a gene on a specified sample signified by columns. There was a clear demarcation between the control samples with most of the genes that are either downregulated (hypermethylated promoters in red) or upregulated (hypomethylated promoters in green) between samples represented in the six rows. The overall methylation clustering between treated and control samples on the top (red and blue) represents quantitative methylation clustering between significant genes.