Huaju Xiaoji Formula Regulates ERS-lncMGC/miRNA to Enhance the Renal Function of Hypertensive Diabetic Mice with Nephropathy

Figure 6

HJXJ intervention decreased ER stress and the expression of the downstream molecules in HGMCs induced by high doses of sugar (30 mmol/mL) and TGFβ1 (5 ng/mL). (a) The mRNA levels of ER stress chaperone genes (CHOP and GRP78) were detected by RT-qPCR analysis. (b) The protein levels of CHOP and GRP78 were measured by Western blotting assays. (c) RT-qPCR was performed to evaluate the expression of ER stress-induced lncMGC, miR379, miR494, miR495, and miR377. (d) The mRNA levels of CUGBP2, CPEB4, EDEM3, and ATF3 were detected by RT-qPCR. (e) The protein levels of CUGBP2, CPEB4, EDEM3, and ATF3 were estimated by Western blotting assays. (f) The histogram analysis of the grey intensity of CUGBP2, CPEB4, EDEM3, and ATF3 was performed. , vs. Con group; ^# and ^##, vs. the STZ-L-NAME group.

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