Research Article
Huaju Xiaoji Formula Regulates ERS-lncMGC/miRNA to Enhance the Renal Function of Hypertensive Diabetic Mice with Nephropathy
Table 1
Primer sequences used in quantitative real-time polymerase chain reaction (RT-qPCR).
| | Gene | Forward primer (5 to 3) | Reverse primer (5 to 3) |
| | CUGBP (mus) | CTATGAACGGCTTTCAGATCGG | GACATGCTAGGCAAACGATGAC | | CPEB4 (mus) | CGCAAGCAATCATATTCAG | CATCGGAAACAATGAAGAC | | EDEM3 (mus) | AAGATGACTGCCCACTCC | GCCGAGATGCCACAATAC | | ATF3 (mus) | GAGTGAAACTGGCTGATG | GGCTGTGGTTATCTTTGG | | GAPDH (mus) | CTGCCCAGAACATCATCC | CTCAGATGCCTGCTTCAC | | miR379–5p (mus) | GCGCGTGGTAGACTATGGAA | AGTGCAGGGTCCGAGGTATT | | miR494–3p (mus) | CGCGTGAAACATACACGGGA | AGTGCAGGGTCCGAGGTATT | | miR495–3p (mus) | GCGAAACAAACATGGTGCA | AGTGCAGGGTCCGAGGTATT | | miR377–3p (mus) | CGCGATCACACAAAGGCAAC | AGTGCAGGGTCCGAGGTATT | | U6 (mus) | GCTTCGGCAGCAC | GGAACGCTTCACG | | CUGBP (homo) | CGTGTTGTATTGTTGGTAG | TTACTGAGGTGAACTGTAG | | CPEB4 (homo) | TCACTGATTGCCTAAACC | ACACAAACACCCATTACC | | EDEM3 (homo) | CTACCAGTGGCCTTCCTTATC | TCAATCCCTGCTCCAACTC | | ATF3 (homo) | TACTCTTCCGATGTTTGTG | CTTTCTTCCTGTGACTTTG | | GAPDH (homo) | AATCCCATCACCATCTTC | AGGCTGTTGTCATACTTC | | miR379–5p (homo) | GCGCGTGGTAGACTATGGAA | AGTGCAGGGTCCGAGGTATT | | miR494–3p (homo) | CGCGTGAAACATACACGGGA | AGTGCAGGGTCCGAGGTATT | | miR495–3p (homo) | GCGAAACAAACATGGTGCA | AGTGCAGGGTCCGAGGTATT | | miR377–3p (homo) | CGCGATCACACAAAGGCAAC | AGTGCAGGGTCCGAGGTATT | | U6 (homo) | CTCGCTTCGGCAGCACA | AACGCTTCACGAATTTGCGT |
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