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Journal of Engineering
Volume 2013 (2013), Article ID 979547, 8 pages
Research Article

Enhanced Production of Cellulase from Pineapple Waste by Response Surface Methodology

Department of Chemical Engineering, Annamalai University, Annamalainagar 608002, Tamilnadu, India

Received 31 August 2012; Revised 5 November 2012; Accepted 11 November 2012

Academic Editor: Tingyue Gu

Copyright © 2013 P. Saravanan et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Optimization of the media components for cellulase production using Trichoderma reesei was carried out. The optimization of cellulase production using pineapple waste as substrate was performed with statistical methodology based on experimental designs. The screening of nutrients and their influence on the cellulase production was studied using a Plackett-Burman design. Avicel, soybean cake flour, KH2PO4, and yeast extract were found to have the positive influence for the production of cellulase. The selected components were optimized using response surface methodology. The optimum concentrations are avicel: 26.5 g/L, soybean cake flour: 22.5 g/L, KH2PO4: 4.5 g/L, and yeast extract: 12.3 g/L. A maximum cellulase activity of 8.61 IU/mL was obtained under the optimized medium in the validation experiment.