Partial Purification and Characterization of the Mode of Action of Enterocin S37: A Bacteriocin Produced by Enterococcus faecalis S37 Isolated from Poultry Feces

Table 2

Effect of enzymes, pH, and heat treatment on antibacterial activity of enterocin S37 against L. monocytogenes EGDe and L. innocua F.

Treatments	Test strains
	L. monocytogenes EGDe	L. innocua F
Enzymes
𝛼-chymotrypsin (1 mg⋅ml^−1)	−	−
Proteinase K (0.5 mg⋅ml^−1)	−	−
Papain (1mg⋅ml^−1)	−	−
Lysozyme (2 mg⋅ml^−1)	+	+
Lipase (1mg⋅ml^−1)	+	+
Assessment of antibacterial stability of enterocin S37 at different pH values
3.0	−	−
4.0	+	+
5.0	+	+
6.0	+	+
7.0	+	+
8.0	+	+
9.0	+	+
10.0	−	−
Antibacterial activity after heating
80^∘C, 15 min	+	+
80^∘C, 60 min	+	+
90^∘C, 15 min	+	+

+: Antibacterial activity detected; −: Absence of antibacterial activity.