Antibiotic Resistance Profile of Bacteria Isolated from Wastewater Systems in Eastern Ethiopia

Table 1

Media and incubation conditions used for the enumeration, and primary isolation of the indicated bacteria from wastewater samples.

Bacteria	Media	Incubation conditions
Total heterotrophic count	R2A agar	37°C; 24 h
Total coliforms	mEndo-LES agar	37°C; 24 h
Fecal coliforms	mFC agar	44.5°C; 24 h
Enterococcus spp. (E. faecalis and E. faecium)	mEnterococcus agar + TITG agar base	37°C; 48 h ← 35–37°C for 18–24 hours
Escherichia coli	m-TEC agar	35–37°C for 2 hours and at 44.5 ± 0.5°C for 22 hours
Aeromonas spp.	mADA-V agar	37°C; 24 h
Pseudomonas aeruginosa	Cetrimide agar	35°C for 18 h