Prevalence and Phenotypic and Genotypic Resistance Mechanisms of Multidrug-Resistant Pseudomonas aeruginosa Strains Isolated from Clinical, Environmental, and Poultry Litter Samples from the Ashanti Region of Ghana

Table 1

Primer nucleotide sequences for detection of antibiotic-resistant genes.

Primer	Primer sequence (5′-3′)	Amplicon size (bp)	Annealing temperature (°C)	Homology	Reference/accession number
oprL-F	ATG GAA ATG CTG AAA TTC GGC	504	64	P. aeruginosa identification	[13]
oprL-R	CTT CTT CAG CTC GAC GCG ACG
5′CS	GGCATCCAAGC GCAAG	Variable	59.5	Conserved region of class 1 integron	[22]
3′CS	AAG CAG ACT TGA CCT GA
Int2-F	CACGGATATGCGACAAAAAGGT	788	60	Class 2 integron	[23]
Int2-R	GTAGCAAACGAGTGACGAAATG
aac(3)-IV -F	GTGTGCTGCTGGTCCACAGC	627	50	Aminoglycoside acetyltransferase	[24]
aac(3)-IV-R	AGTTGACCCAGGGCTGTCGC
SHV 1	GGG TTA TTC TTA TTT GTC GC	900	56	SHV ESBL	[25]
SHV 2	TTA GCG TTG CCA GTG CTC
TEM1-F	ATG AGT ATT CAA CAT TTC CG	867	58	TEM ESBL	[25]
TEM1-R	CTG ACA GTT ACC AAT GCT TA
CTMX-F	ATG TGC AGY ACC AGT AAR GTK ATG GC	593	60	CTMX ESBL	[26]
CTMX -R	TGG GTR AAR TAR GTS ACC AGA AYC AGC G
VIM-F	ATG GTG TTT GGT CGC ATA TC	261	51	VIM MBL	[25]
VIM -R	TGG GCC ATT CAG CCA GAT C
IMP -F	CTA CCG CAG AGT CTT TG	600	55	IMP MBL	[25]
IMP- R	AAC CAG TTT TGC CTT ACC AT