Green Coffee Bean Extract Potentially Ameliorates Liver Injury due to HFD/STZ-Induced Diabetes in Rats

Table 2

The primers’ sequences.

Gene	Primer sequence (5′–3′)	Reference	Gene accession number
GAPDH reference gene	Sense: 5′GCA​TCT​TCT​TGT​GCA​GTG​CC-3′	GenBank primer-blast program, NCBI	NM_017008.4
	Antisense: 5′ GAT​GGT​GAT​GGG​TTT​CCC​GT-3′
Glucokinase (GK)	Sense: 5′GTG​GTG​CTT​TTG​AGA​CCC​GTT-3′	GenBank primer-blast program, NCBI	NM_001270850.1
	Antisense: 5′-TTC​GAT​GAA​GGT​GAT​TTC​GCA-3′
Hexokinase (HK)	Sense: 5′-AGG​GGG​ATT​TCA​TTG​CCC​TG-3′	GenBank primer-blast program, NCBI	NM_012734.1
	Antisense: 5′-TCT​TCT​CGT​GGT​TCA​CCT​GC-3′
Glucose-6- phosphate dehydrogenase (G6PD)	Sense: 5′-GTA​TGT​GGG​GAA​CCC​CAG​TG-3′	GenBank primer-blast program, NCBI	NM_017006.2
	Antisense: 5′-AGG​GAC​AGA​CTG​ATA​GGC​GT-3′
Pyruvate kinase (PK)	Sense: 5′-CCT​CTG​CCT​TCT​GGA​TAC​TGA​CT-3′	GenBank primer-blast program, NCBI	NM_012624.3
	Antisense: 5′-GCA​AGA​CTC​CGG​TTC​GTA​TCT-3′
Fatty acid synthase (FASN)	Sense: 5′-GCT​TGG​TGA​ACT​GTC​TCC​GA-3′	GenBank primer-blast program, NCBI	NM_017332.1
	Antisense: 5′-GGG​TCC​AGC​TAG​AGG​GTA​CA-3′
Phosphoinositide-3-kinase, regulatory subunit 2 (beta) (PIK3R2)	Sense: 5′-TAC​CAG​CAC​GCA​TCA​CTT​GT-3′;	GenBank primer-blast program, NCBI	NM_022185.2;
	Antisense: 5′-ATG​GCT​TGA​AGA​ACT​CGG​GG-3′
Insulin receptor (INSR)	Sense: 5′-GTT​TTT​GTT​CCC​AGG​CCA​TCC3′;	GenBank primer-blast program, NCBI	NM_006248753.2
	Antisense: 5′AAT​GCT​TCC​GGG​AGA​CAC​AG-3′
Insulin receptor substrate 1 (IRS1)	Sense: 5′CTG​CAT​AAT​CGG​GCA​AAG​GC3′	GenBank primer-blast program, NCBI	NM_012969.1
	Antisense: 5′CAT​CGC​TAG​GAG​AAC​CGG​AC3′
Peroxisome proliferator-activated receptor α (PPARα)	Sense: 5′TAT​TTA​TTG​CAT​CCA​GTG​GG3′	GenBank primer-blast program, NCBI	NM_013196.2
	Antisense: 5′ATG​TTT​CCC​ATC​TCT​TGT​AA3′