Aqueous Extract of Sparganii Rhizoma and Curcumae Rhizoma Induces Apoptosis and Inhibits Migration in Human Oral Squamous Cell Carcinoma

Figure 3

SRCR induced ROS production and mitochondrial dysfunction in human OSCC cells. (a-b) After incubating SCC4 and HSC3 cells with various concentrations of SRCR for 1 h, ROS generation was assessed via CM-H₂DCFDA staining in conjunction with flow cytometric analysis; (c-d) after pretreating cells with catalase (10,000 U/ml) and NAC (5 μM) for 30 min followed by stimulation using SRCR (240 μg/mL) for 48 h, cell viability was examined via a CCK-8 assay; (e–h) after incubating SCC4 and HSC3 cells with various concentrations of SRCR for 24 h, MMP was examined via flow cytometry with JC-1 staining; (i-j) after treating cells with different concentrations of SRCR for 24 h, the expression levels of Bcl-2, Bcl-xL, Bak, and Bax were determined using western blot analysis. Results are expressed as mean ± SD of four independent experiments. compared to the control group; ^# compared to the SRCR-treated group.

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