Aqueous Extract of Sparganii Rhizoma and Curcumae Rhizoma Induces Apoptosis and Inhibits Migration in Human Oral Squamous Cell Carcinoma

Figure 4

SRCR induced Ca²⁺ release and activation of ER stress in human OSCC cells. (a-b) After incubating SCC4 and HSC3 cells with SRCR for 5 h, Ca²⁺ flux was assessed via flow cytometry; (c-d) after incubating cells with SRCR for 24 h, the expression of GRP78, GRP94, calpain I, and calpain II was assessed via western blot analysis; (e-f) after pretreating cells with BAPTA-AM (10 μM) for 30 min and SRCR (240 μg/mL) for 48 h, cell proliferation was analyzed via a CCK-8 assay. Results are expressed as mean ± SD. compared to the control group; ^# compared to the SRCR-treated group.

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