Study on Antipyretic Properties of Phenolics in Lonicerae Japonicae Flos Based on Ultrahigh Performance Liquid Chromatography-Tandem Mass Spectrometry Combined with Network Pharmacology
Table 2
Regression equation, detection limit, quantification limit, repeatability, precision, stability, and sample recovery rate of quantitative components.
No.
Calibration curve
R2
Linearity range (μg/mL)
LOD (ng/mL)
LOQ (ng/mL)
Repeatability
Precision
Stability
Recovery
RSD (%)
Intraday RSD (%)
Interday RSD (%)
RSD (%)
Original (%)
RSD (%)
P1
y = 6662.6x + 5980.2
0.9998
0.5950-23.8000
0.50
1.68
1.58
0.98
0.99
1.75
100.17
0.83
P2
y = 10927x + 571.11
0.9992
0.3875-15.5000
0.53
1.76
1.76
0.46
0.83
1.96
99.94
0.73
P3
y = 3886.2x + 750.51
0.9997
0.2650-10.6000
0.52
1.74
1.43
0.59
0.95
1.77
100.34
1.11
P4
y = 1981.6x + 281.4
0.9992
0.0216–0.8630
0.37
1.24
1.75
0.78
3.44
2.15
100.36
2.16
P5
y = 2271.9x + 189.6
0.9995
0.0128–0.5130
0.37
1.24
1.67
0.98
2.11
2.87
100.20
2.82
P6
y = 3030.5x + 171.1
0.9997
0.0116–0.4630
0.46
1.52
1.89
1.21
2.35
1.96
99.77
2.46
P7
y = 2100.2x + 139.6
0.9992
0.0750–3.0000
0.50
1.68
1.45
0.65
2.01
1.94
100.04
1.19
P8
y = 796.4x + 123.6
0.9993
0.1131–4.5250
0.49
1.64
1.65
0.57
2.91
1.87
99.92
0.50
P9
y = 718.73x + 112.92
0.9998
0.4525-18.1000
0.52
1.74
1.43
0.88
3.32
2.32
99.77
0.99
P10
y = 3301.8x + 130.6
0.9991
0.0113–0.4500
0.38
1.28
1.64
0.78
3.27
1.88
99.34
0.42
P11
y = 790.51x + 38.4
0.9998
0.0069–0.2750
0.50
1.68
1.85
0.89
1.72
2.09
98.58
2.63
P12
y = 4491.6x + 81.3
0.9996
0.0026–0.1050
0.38
1.28
1.35
1.32
2.05
2.87
102.14
1.89
F1
y = 3269.9x + 1928.8
0.9990
0.2452-49.0447
0.57
1.91
2.92
2.98
3.31
3.47
98.41
3.05
F2
y = 10869x + 569.38
0.9997
0.0339–6.7830
0.10
0.34
1.44
1.78
2.48
0.77
103.40
1.01
F3
y = 6888.2x + 4159.7
0.9994
0.1228-24.5568
0.33
1.10
3.02
2.52
3.26
2.81
96.91
1.42
F4
y = 5228.3x + 4981.8
0.9994
0.1185-23.7092
0.13
0.45
2.58
1.87
2.74
2.01
99.03
1.03
F5
y = 2404.9x + 2990.9
0.9995
0.2136-42.7217
0.38
1.26
1.99
1.69
1.44
2.89
92.80
2.34
F6
y = 6499.5x + 81.474
1
0.0533-10.6518
0.09
0.28
1.06
0.16
1.12
1.15
102.10
1.18
F7
y = 23410x + 21.278
1
0.0099–1.9803
0.02
0.06
0.57
0.34
1.13
0.75
107.40
1.53
F8
y = 4099x + 55.215
0.9997
0.0095–1.9050
0.06
0.21
1.04
1.38
2.20
1.61
94.28
1.81
F9
y = 10296x + 541.59
0.9997
0.0358–7.1690
0.27
0.90
1.53
1.27
3.03
3.20
98.19
0.46
F10
y = 3363.5x + 41.835
0.9999
0.0137–2.7367
0.08
0.26
0.92
1.03
1.84
2.25
103.60
1.17
F11
y = 7915.4x + 2129.1
0.9993
0.0997-19.9367
0.16
0.55
3.01
2.97
3.71
3.38
103.80
3.04
F12
y = 2694.1x + 19.246
0.9994
0.0044–0.8891
0.03
0.11
1.46
3.17
3.19
1.74
101.00
2.55
F13
y = 32037x − 27.074
1
0.0017–0.3481
0.01
0.04
2.97
2.14
3.01
2.80
97.31
2.54
F14
y = 579.2x − 3.4908
1
0.0064–1.2717
0.09
0.29
0.55
0.74
2.24
1.79
95.99
1.04
F15
y = 15697x + 34.838
0.9999
0.0045–0.8975
0.04
0.12
1.46
1.09
1.13
2.38
103.80
2.01
Note. The components corresponding to the numbers in the table are the same as in Table 1.
