Interaction and Binding Kinetics of Different Substituted Pyrazines with HSA: Based on Multispectral, Physiological Activity, and Molecular Dynamics Simulations

Figure 2

Fluorescence quenching spectra of different pyrazines and HSA: (a) 2,3-DMTP, (b) 2,5-DMTP, (c) TMTP, and (d) TTMP.  = 1.0 × 10⁻⁴ mol·L⁻¹, T = 301 K, and (A–J): 0, 7.5, 15, 22.5, 30, 37.5, 45, 52.5, 60, 67.5 (×10⁻⁶ mol·L⁻¹).

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(b)

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