Interaction and Binding Kinetics of Different Substituted Pyrazines with HSA: Based on Multispectral, Physiological Activity, and Molecular Dynamics Simulations

Table 1

Quenching constants of HSA at different temperatures.

	T (K)	(L·mol^−1)	(L·mol^−1·s^−1)	R
2,3-DMTP	291	6.332 × 10^3	6.332 × 10^11	0.9920
	301	5.865 × 10^3	5.865 × 10^11	0.9979
	310	5.656 × 10^3	5.656 × 10^11	0.9905
2,5-DMTP	291	5.315 × 10^3	5.315 × 10^11	0.9662
	301	4.820 × 10^3	4.820 × 10^11	0.9853
	310	4.139 × 10^3	4.139 × 10^11	0.9924
TMTP	291	1.059 × 10^4	1.059 × 10^12	0.9928
	301	9.778 × 10^3	9.778 × 10^11	0.9989
	310	9.319 × 10^3	9.319 × 10^11	0.9903
TTMP	291	2.029 × 10^4	2.029 × 10^12	0.9978
	301	1.924 × 10^4	1.924 × 10^12	0.9973
	310	1.855 × 10^4	1.855 × 10^12	0.9970