Journal of Healthcare Engineering

Journal of Healthcare Engineering / 2010 / Article

Research Article | Open Access

Volume 1 |Article ID 538548 | 12 pages |

Selective Inactivation of Viruses with Femtosecond Laser Pulses and its Potential Use for in Vitro Therapy


Introduction: Traditional biochemical and pharmaceutical methods employed today encounter problems of clinical side effects and drug resistance, and their use is becoming limited. Therefore, it has become important and necessary to develop new, alternative strategies to combat viral diseases.Materials and Method: A variety of viruses including M13 bacetriophage (nonenveloped ssDNA), tobacco mosaic virus (nonenveloped ssRNA), human papillomavirus (nonenveloped dsDNA) and human immunodeficiency virus (enveloped ssRNA), together with human red blood cells, Jurkat T-cells and mouse dendritic cells in their buffer solutions have been irradiated with near-infrared subpicosecond laser pulses in vitro.Results: A window of laser power density, approximately between 1 GW/cm2 and 10 GW/cm2, has been observed that allows killing the viral particles while leaving mammalian cells unharmed.Conclusion: The ultrashort pulsed laser technology may have great potential for disinfection of blood components.


  1. K. Rosenheck and P. Doty, “The far ultraviolet absorption spectra of polypeptide and protein solutions and their dependence on conformation,” Proc Natl. Acad. Sci. U S A, vol. 47, no. 11, pp. 1775–1785, 1961. View at: Google Scholar
  2. J. C. Sutherland and K. P. Griffin, “Absorption spectrum of DNA for wavelengths greater than 300 nm,” Radiation Research, vol. 86, 3990410, 1981. View at: Google Scholar
  3. B. J. Bryant and H. G. Klein, “Pathogen Inactivation: The Definitive Safeguard for the Blood Supply,” Arch. Pathol. Lab. Med., vol. 131, pp. 719–733, 2007. View at: Google Scholar
  4. K. T. Tsen, S.-W. D. Tsen, C.-L. Chang, C.-F. Hung, T. C. Wu, and J. G. Kiang, “Inactivation of viruses by coherent excitations with a low power visible femtosecond laser,” Virology J., vol. 4, no. 50, pp. 1–5, 2007. View at: Google Scholar
  5. K. T. Tsen, S.-W D. Tsen, C.-L. Chang, C.-F. Hung, T. C. Wu, and J. G. Kiang, “Inactivation of viruses by laser-driven coherent excitations via impulsive stimulated Raman scattering process,” J. Biomedical Optics, vol. 12, no. 1–6, 064030, 2007. View at: Google Scholar
  6. K. T. Tsen, S.-W. D. Tsen, C.-L. Chang, C.-F. Hung, T. C. Wu, and J. G. Kiang, “Inactivation of viruses with a very low power visible femtosecond laser,” J. Physics: Condensed Matter, vol. 19, no. 1–9, 322102, 2007. View at: Google Scholar
  7. K. T. Tsen, S.-W. D. Tsen, O. F. Sankey, and J. G. Kiang, “Selective inactivation of microorganisms with near-infrared femtosecond laser pulses,” J Phys: Condensed Matter, vol. 19, no. 1–7, 472201, 2007. View at: Google Scholar
  8. K. T. Tsen, Shaw-Wei D Tsen, Chien-Fu Hung, T.-C. Wu, and Juliann G Kiang, “Selective inactivation of human immunodeficiency virus with subpicosecond near-infrared laser pulses,” J. Phys.: Condensed Matter, vol. 20, no. 1–7, 252205, 2008. View at: Google Scholar
  9. K. T. Tsen, Shaw-Wei D. Tsen, Q. Fu et al., “Photonic approach to the selective inactivation of viruses with a near-infrared subpicosecond fiber laser,” J. Biomedical Optics, vol. 14, no. 1–7, 064042, 2009. View at: Google Scholar
  10. A. Miyanohara and K. Bouic, 2005,
  11. Constructs and detailed protocols for the preparation of the pseudovirions can be found online at
  12. T. Mosmann, J. Immunol. Methods, vol. 65, p. 55, 1983.
  13. Y.-X. Yan, E. B. Gamble Jr., and Keith A. Nelson, “Impulsive stimulated scattering: General importance in femtosecond laser pulse interactions with matter, and spectroscopic applications,” J. Chem. Phys., vol. 83, pp. 5391–5399, 1985. View at: Google Scholar
  14. K. A. Nelson, R. J. D. Miller, D. R. Lutz, and M. D. Fayer, “Optical generation of tunable ultrasonic waves,” J. Appl. Phys., vol. 53, pp. 1144–1149, 1982. View at: Google Scholar
  15. S. De Silvestri, J. G. Fugimoto, E. P. Ippen, E. B. Gamble Jr., L. R. Williams, and K. A. Nelson, “Femtosecond time-resolved measurements of optic phonon dephasing by impulsive stimulated raman scattering in α-perylene crystal from 20 to 300 K,” Chem. Phys. Lett., vol. 116, pp. 146–152, 1985. View at: Google Scholar
  16. K. A. Nelson, “Stimulated Brillouin scattering and optical excitation of coherent shear Waves,” J. Appl. Phys., vol. 53, pp. 6060–6063, 1982. View at: Google Scholar
  17. G. C. Cho, W. Kutt, and H. Kurz, “Subpicosecond time-resolved coherent-phonon oscillations in GaAs,” Phys. Rev. Lett., vol. 65, pp. 764–766, 1990. View at: Google Scholar
  18. T. K. Cheng, J. Vidal, H. J. Zeiger, G. Dresselhaus, M. S. Dresselhaus, and E. P. Ippen, “Mechanism for displacive excitation of coherent phonons in Sb, Bi, Te, and Ti2O3,” Appl. Phys. Lett., vol. 59, pp. 1923–1925, 1991. View at: Google Scholar
  19. J. M. Chwalek, C. Uher, J. F. Whittaker, and G. A. Mourou, “Subpicosecond time-resolved studies of coherent phonon oscillations in thin-film YBa2Cu3O6+x (x<0.4),” Appl. Phys. Lett., vol. 58, pp. 980–982, 1991. View at: Google Scholar
  20. R. Merlin, “Generating coherent THz phonons with light pulses,” Solid State Communications, vol. 102, pp. 207–220, 1997. View at: Google Scholar
  21. M. Boustie, L. Berthe, T. de Resseguier, and M. Arrigoni, “Laser shock waves: fundamentals and applications,” in Proc. 1st Int. Symp. on Laser Ultrasonics: Science, Technology and Applications, paper #2, National Research Council of Canada, Montreal, 2008. View at: Google Scholar
  22. L. T. Goodnough, “Risks of blood transfusion,” Anesthesiology clinics of North America, vol. 23, no. 2, pp. 241–252, v (2005). View at: Google Scholar

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