Research Article | Open Access
Selective Inactivation of Viruses with Femtosecond Laser Pulses and its Potential Use for in Vitro Therapy
Introduction: Traditional biochemical and pharmaceutical methods employed today encounter problems of clinical side effects and drug resistance, and their use is becoming limited. Therefore, it has become important and necessary to develop new, alternative strategies to combat viral diseases.Materials and Method: A variety of viruses including M13 bacetriophage (nonenveloped ssDNA), tobacco mosaic virus (nonenveloped ssRNA), human papillomavirus (nonenveloped dsDNA) and human immunodeficiency virus (enveloped ssRNA), together with human red blood cells, Jurkat T-cells and mouse dendritic cells in their buffer solutions have been irradiated with near-infrared subpicosecond laser pulses in vitro.Results: A window of laser power density, approximately between 1 GW/cm2 and 10 GW/cm2, has been observed that allows killing the viral particles while leaving mammalian cells unharmed.Conclusion: The ultrashort pulsed laser technology may have great potential for disinfection of blood components.
- K. Rosenheck and P. Doty, “The far ultraviolet absorption spectra of polypeptide and protein solutions and their dependence on conformation,” Proc Natl. Acad. Sci. U S A, vol. 47, no. 11, pp. 1775–1785, 1961.
- J. C. Sutherland and K. P. Griffin, “Absorption spectrum of DNA for wavelengths greater than 300 nm,” Radiation Research, vol. 86, 3990410, 1981.
- B. J. Bryant and H. G. Klein, “Pathogen Inactivation: The Definitive Safeguard for the Blood Supply,” Arch. Pathol. Lab. Med., vol. 131, pp. 719–733, 2007.
- K. T. Tsen, S.-W. D. Tsen, C.-L. Chang, C.-F. Hung, T. C. Wu, and J. G. Kiang, “Inactivation of viruses by coherent excitations with a low power visible femtosecond laser,” Virology J., vol. 4, no. 50, pp. 1–5, 2007.
- K. T. Tsen, S.-W D. Tsen, C.-L. Chang, C.-F. Hung, T. C. Wu, and J. G. Kiang, “Inactivation of viruses by laser-driven coherent excitations via impulsive stimulated Raman scattering process,” J. Biomedical Optics, vol. 12, no. 1–6, 064030, 2007.
- K. T. Tsen, S.-W. D. Tsen, C.-L. Chang, C.-F. Hung, T. C. Wu, and J. G. Kiang, “Inactivation of viruses with a very low power visible femtosecond laser,” J. Physics: Condensed Matter, vol. 19, no. 1–9, 322102, 2007.
- K. T. Tsen, S.-W. D. Tsen, O. F. Sankey, and J. G. Kiang, “Selective inactivation of microorganisms with near-infrared femtosecond laser pulses,” J Phys: Condensed Matter, vol. 19, no. 1–7, 472201, 2007.
- K. T. Tsen, Shaw-Wei D Tsen, Chien-Fu Hung, T.-C. Wu, and Juliann G Kiang, “Selective inactivation of human immunodeficiency virus with subpicosecond near-infrared laser pulses,” J. Phys.: Condensed Matter, vol. 20, no. 1–7, 252205, 2008.
- K. T. Tsen, Shaw-Wei D. Tsen, Q. Fu et al., “Photonic approach to the selective inactivation of viruses with a near-infrared subpicosecond fiber laser,” J. Biomedical Optics, vol. 14, no. 1–7, 064042, 2009.
- A. Miyanohara and K. Bouic, 2005, http://www.virapur.com/?page-id=41.
- Constructs and detailed protocols for the preparation of the pseudovirions can be found online at http://home.ccr.cancer.gov/lco/default.asp.
- T. Mosmann, J. Immunol. Methods, vol. 65, p. 55, 1983.
- Y.-X. Yan, E. B. Gamble Jr., and Keith A. Nelson, “Impulsive stimulated scattering: General importance in femtosecond laser pulse interactions with matter, and spectroscopic applications,” J. Chem. Phys., vol. 83, pp. 5391–5399, 1985.
- K. A. Nelson, R. J. D. Miller, D. R. Lutz, and M. D. Fayer, “Optical generation of tunable ultrasonic waves,” J. Appl. Phys., vol. 53, pp. 1144–1149, 1982.
- S. De Silvestri, J. G. Fugimoto, E. P. Ippen, E. B. Gamble Jr., L. R. Williams, and K. A. Nelson, “Femtosecond time-resolved measurements of optic phonon dephasing by impulsive stimulated raman scattering in α-perylene crystal from 20 to 300 K,” Chem. Phys. Lett., vol. 116, pp. 146–152, 1985.
- K. A. Nelson, “Stimulated Brillouin scattering and optical excitation of coherent shear Waves,” J. Appl. Phys., vol. 53, pp. 6060–6063, 1982.
- G. C. Cho, W. Kutt, and H. Kurz, “Subpicosecond time-resolved coherent-phonon oscillations in GaAs,” Phys. Rev. Lett., vol. 65, pp. 764–766, 1990.
- T. K. Cheng, J. Vidal, H. J. Zeiger, G. Dresselhaus, M. S. Dresselhaus, and E. P. Ippen, “Mechanism for displacive excitation of coherent phonons in Sb, Bi, Te, and Ti2O3,” Appl. Phys. Lett., vol. 59, pp. 1923–1925, 1991.
- J. M. Chwalek, C. Uher, J. F. Whittaker, and G. A. Mourou, “Subpicosecond time-resolved studies of coherent phonon oscillations in thin-film YBa2Cu3O6+x (x<0.4),” Appl. Phys. Lett., vol. 58, pp. 980–982, 1991.
- R. Merlin, “Generating coherent THz phonons with light pulses,” Solid State Communications, vol. 102, pp. 207–220, 1997.
- M. Boustie, L. Berthe, T. de Resseguier, and M. Arrigoni, “Laser shock waves: fundamentals and applications,” in Proc. 1st Int. Symp. on Laser Ultrasonics: Science, Technology and Applications, paper #2, National Research Council of Canada, Montreal, 2008.
- L. T. Goodnough, “Risks of blood transfusion,” Anesthesiology clinics of North America, vol. 23, no. 2, pp. 241–252, v (2005).
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