Model of Murine Ventricular Cardiac Tissue for In Vitro Kinematic-Dynamic Studies of Electromagnetic and β-Adrenergic Stimulation

Figure 5

Immunofluorescence. A Fire LUT was applied using ImageJ in order to show the levels of MF20 immunofluorescence after 48 h of culture (white scale bar, 10 μm; color scale in the range of 0–256 [arbitrary unit]). In comparison with control (CTRL), the isoproterenol (ISO) showed an enhancement of the fluorescence, whereas the electromagnetic stimulation (EMF) caused a reduction. The simultaneous use of pharmacological and physical stimulation (ISO + EMF) weakened the effect of ISO, giving an impairment in comparison with CTRL. The physically stimulated cultures showed an antisarcomeric effect of the electromagnetic field in the long term.