Research Article

Gene Expression Changes in Long-Term In Vitro Human Blood-Brain Barrier Models and Their Dependence on a Transwell Scaffold Material

Table 2

Significant differences in gene expression between the biopaper and PET coculture systems at each time point. Genes with significantly higher expression on the biopaper and PET are shown in italic and bold formats, respectively. Genes with no expression on the biopaper or PET at any time are presented with dashes.

Day 3Day 7Day 14Day 21Day 28

Extracellular matrix-associated proteinsSPARC
COL4A1
LAMA1
LAMA2
LAMA4
AGRN

Functional astrocyte proteinsGFAP
AQP4

Membrane transporters and soluble factorsPDGFB
PDGFRB
GGT5
LIF
FGF2

Endothelial tight junction transmembrane proteinsOCLN
CLDN1
CLDN3
CLDN5
CLDN12

Endothelial adherens junction transmembrane proteinsCDH5

Endothelial junction accessory proteinsTJP1
TJP2
TJP3

Gap junction proteinsGJA1
GJB4
GJB6

Cell adhesion proteinsVCAM1
PECAM1