Accurate Rapid Lifetime Determination on Time-Gated FLIM Microscopy with Optical Sectioning

Table 1

Fluorescence lifetimes measured for Coumarin 153 and Erythrosine B solutions in capillary tubes using the RLD technique on epi-fluorescence and HiLo images. The measured values correspond to mean ± standard deviation of the intensity-weighted fluorescence lifetime image. The reference lifetimes were obtained with a time-correlated single-photon counting microscope.

Solution	Reference lifetime (ps)	Epi-fluorescence image lifetime (ps)	HiLo image lifetime (ps)
Coumarin 153	3959.7	3825.6 ± 198.1	3846.6 ± 463.5
Erythrosine B	490.7	604.2 ± 40.4	613.2 ± 42.0