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Journal of Healthcare Engineering
Volume 2018, Article ID 6573947, 13 pages
https://doi.org/10.1155/2018/6573947
Research Article

Potential of Electrospun Poly(3-hydroxybutyrate)/Collagen Blends for Tissue Engineering Applications

1Department of Engineering for Innovation, University of Salento, Campus Ecotekne, Via per Monteroni, 73100 Lecce, Italy
2Distretto Tecnologico High Tech DHITECH Scarl, Campus Ecotekne, Via per Monteroni, 73100 Lecce, Italy
3EggPlant Srl, Via Don Minzoni 27, 70044 Polignano a Mare, Italy
4Department of Biomedical Sciences and Human Oncology, University of Bari “Aldo Moro”, Piazza Giulio Cesare 11, 70124 Bari, Italy
5Institute of Nanotechnology of the National Research Council (CNR NANOTEC), CNR, Campus Ecotekne, Via per Monteroni, 73100 Lecce, Italy

Correspondence should be addressed to Marta Madaghiele; ti.otnelasinu@eleihgadam.atram

Received 15 January 2018; Accepted 28 March 2018; Published 19 April 2018

Academic Editor: Saverio Affatato

Copyright © 2018 Luca Salvatore et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. J. A. Matthews, G. E. Wnek, D. G. Simpson, and G. L. Bowlin, “Electrospinning of collagen nanofibers,” Biomacromolecules, vol. 3, no. 2, pp. 232–238, 2002. View at Publisher · View at Google Scholar · View at Scopus
  2. L. Buttafoco, N. G. Kolkman, P. Engbers-Buijtenhuijs et al., “Electrospinning of collagen and elastin for tissue engineering applications,” Biomaterials, vol. 27, no. 5, pp. 724–734, 2006. View at Publisher · View at Google Scholar · View at Scopus
  3. Y. Ji, K. Ghosh, X. Z. Shu et al., “Electrospun three-dimensional hyaluronic acid nanofibrous scaffolds,” Biomaterials, vol. 27, no. 20, pp. 3782–3792, 2006. View at Publisher · View at Google Scholar · View at Scopus
  4. L. Meng, O. Arnoult, M. Smith, and G. E. Wnek, “Electrospinning of in situ crosslinked collagen nanofibers,” Journal of Materials Chemistry, vol. 22, no. 37, pp. 19412–19417, 2012. View at Publisher · View at Google Scholar · View at Scopus
  5. R. L. Fischer, M. G. McCoy, and S. A. Grant, “Electrospinning collagen and hyaluronic acid nanofiber meshes,” Journal of Materials Science: Materials in Medicine, vol. 23, no. 7, pp. 1645–1654, 2012. View at Publisher · View at Google Scholar · View at Scopus
  6. A. Fiorani, C. Gualandi, S. Panseri et al., “Comparative performance of collagen nanofibers electrospun from different solvents and stabilized by different crosslinkers,” Journal of Materials Science: Materials in Medicine, vol. 25, no. 10, pp. 2313–2321, 2014. View at Publisher · View at Google Scholar · View at Scopus
  7. W. Meng, S.-Y. Kim, J. Yuan et al., “Electrospun PHBV/collagen composite nanofibrous scaffolds for tissue engineering,” Journal of Biomaterials Science, Polymer Edition, vol. 18, no. 1, pp. 81–94, 2007. View at Publisher · View at Google Scholar · View at Scopus
  8. D. I. Zeugolis, S. T. Khew, E. S. Y. Yew et al., “Electro-spinning of pure collagen nano-fibres – just an expensive way to make gelatin?” Biomaterials, vol. 29, no. 15, pp. 2293–2305, 2008. View at Publisher · View at Google Scholar · View at Scopus
  9. V. Y. Chakrapani, A. Gnanamani, V. R. Giridev, M. Madhusoothanan, and G. Sekaran, “Electrospinning of type I collagen and PCL nanofibers using acetic acid,” Journal of Applied Polymer Science, vol. 125, no. 4, pp. 3221–3227, 2012. View at Publisher · View at Google Scholar · View at Scopus
  10. M. P. Prabhakaran, E. Vatankhah, and S. Ramakrishna, “Electrospun aligned PHBV/collagen nanofibers as substrates for nerve tissue engineering,” Biotechnology and Bioengineering, vol. 110, no. 10, pp. 2775–2784, 2013. View at Publisher · View at Google Scholar · View at Scopus
  11. C. N. Grover, R. W. Farndale, S. M. Best, and R. E. Cameron, “The interplay between physical and chemical properties of protein films affects their bioactivity,” Journal of Biomedical Materials Research Part A, vol. 100A, no. 9, pp. 2401–2411, 2012. View at Publisher · View at Google Scholar · View at Scopus
  12. C. N. Grover, J. H. Gwynne, N. Pugh et al., “Crosslinking and composition influence the surface properties, mechanical stiffness and cell reactivity of collagen-based films,” Acta Biomaterialia, vol. 8, no. 8, pp. 3080–3090, 2012. View at Publisher · View at Google Scholar · View at Scopus
  13. X. Qiao, S. J. Russell, X. Yang, G. Tronci, and D. J. Wood, “Compositional and in vitro evaluation of nonwoven type I collagen/poly-dl-lactic acid scaffolds for bone regeneration,” Journal of Functional Biomaterials, vol. 6, no. 3, pp. 667–686, 2015. View at Publisher · View at Google Scholar
  14. G. G. Genchi, G. Ciofani, A. Polini et al., “PC12 neuron-like cell response to electrospun poly( 3-hydroxybutyrate) substrates,” Journal of Tissue Engineering and Regenerative Medicine, vol. 9, no. 2, pp. 151–161, 2015. View at Publisher · View at Google Scholar · View at Scopus
  15. G. Ramanathan, S. Singaravelu, M. D. Raja et al., “Fabrication and characterization of a collagen coated electrospun poly(3-hydroxybutyric acid)–gelatin nanofibrous scaffold as a soft bio-mimetic material for skin tissue engineering applications,” RSC Advances, vol. 6, no. 10, pp. 7914–7922, 2016. View at Publisher · View at Google Scholar · View at Scopus
  16. R. Chen, L. Qiu, Q. Ke, C. He, and X. Mo, “Electrospinning thermoplastic polyurethane-contained collagen nanofibers for tissue-engineering applications,” Journal of Biomaterials Science, Polymer Edition, vol. 20, no. 11, pp. 1513–1536, 2009. View at Publisher · View at Google Scholar · View at Scopus
  17. Z. Chen, X. Mo, and F. Qing, “Electrospinning of collagen–chitosan complex,” Materials Letters, vol. 61, no. 16, pp. 3490–3494, 2007. View at Publisher · View at Google Scholar · View at Scopus
  18. J. Zhou, C. Cao, X. Ma, and J. Lin, “Electrospinning of silk fibroin and collagen for vascular tissue engineering,” International Journal of Biological Macromolecules, vol. 47, no. 4, pp. 514–519, 2010. View at Publisher · View at Google Scholar · View at Scopus
  19. E. Masaeli, M. Morshed, M. H. Nasr-Esfahani et al., “Fabrication, characterization and cellular compatibility of poly(hydroxy alkanoate) composite nanofibrous scaffolds for nerve tissue engineering,” PLoS One, vol. 8, no. 2, article e57157, 2013. View at Publisher · View at Google Scholar · View at Scopus
  20. M. Koller, A. Atlić, M. Dios, A. Reiterer, and G. Braunegg, “Microbial PHA production from waste raw materials,” in Plastic from Bacteria. Microbiology Monographs, G. Q. Chen, Ed., vol. 14, pp. 85–119, Springer, Berlin Heidelberg, 2010. View at Publisher · View at Google Scholar
  21. A. S. Asran, K. Razghandi, N. Aggarwal, G. H. Michler, and T. Groth, “Nanofibers from blends of polyvinyl alcohol and polyhydroxy butyrate as potential scaffold material for tissue engineering of skin,” Biomacromolecules, vol. 11, no. 12, pp. 3413–3421, 2010. View at Publisher · View at Google Scholar · View at Scopus
  22. Q. Wu, Y. Wang, and G.-Q. Chen, “Medical application of microbial biopolyesters polyhydroxyalkanoates,” Artificial Cells, Blood Substitutes, and Biotechnology, vol. 37, no. 1, pp. 1–12, 2009. View at Publisher · View at Google Scholar · View at Scopus
  23. C. J. Brigham and A. J. Sinskey, “Applications of polyhydroxyalkanoates in the medical industry,” International Journal of Biotechnology for Wellness Industries, vol. 1, pp. 53–60, 2012. View at Publisher · View at Google Scholar
  24. G.-Q. Chen and Q. Wu, “The application of polyhydroxyalkanoates as tissue engineering materials,” Biomaterials, vol. 26, no. 33, pp. 6565–6578, 2005. View at Publisher · View at Google Scholar · View at Scopus
  25. K. Sombatmankhong, N. Sanchavanakit, P. Pavasant, and P. Supaphol, “Bone scaffolds from electrospun fiber mats of poly(3-hydroxybutyrate), poly(3-hydroxybutyrate-co-3-hydroxyvalerate) and their blend,” Polymer, vol. 48, no. 5, pp. 1419–1427, 2007. View at Publisher · View at Google Scholar · View at Scopus
  26. E. I. Shishatskaya and T. G. Volova, “A comparative investigation of biodegradable polyhydroxyalkanoate films as matrices for in vitro cell cultures,” Journal of Materials Science: Materials in Medicine, vol. 15, no. 8, pp. 915–923, 2004. View at Publisher · View at Google Scholar · View at Scopus
  27. T. H. Ying, D. Ishii, A. Mahara et al., “Scaffolds from electrospun polyhydroxyalkanoate copolymers: fabrication, characterization, bioabsorption and tissue response,” Biomaterials, vol. 29, no. 10, pp. 1307–1317, 2008. View at Publisher · View at Google Scholar · View at Scopus
  28. L. Ghasemi-Mobarakeh, D. Semnani, and M. Morshed, “A novel method for porosity measurement of various surface layers of nanofibers mat using image analysis for tissue engineering applications,” Journal of Applied Polymer Science, vol. 106, no. 4, pp. 2536–2542, 2007. View at Publisher · View at Google Scholar · View at Scopus
  29. I. Campoy, M. A. Gomez, and C. Marco, “Structure and thermal properties of blends of nylon 6 and a liquid crystal copolyester,” Polymer, vol. 39, no. 25, pp. 6279–6288, 1998. View at Publisher · View at Google Scholar · View at Scopus
  30. K. H. Jones and J. A. Senft, “An improved method to determine cell viability by simultaneous staining with fluorescein diacetate-propidium iodide,” Journal of Histochemistry & Cytochemistry, vol. 33, no. 1, pp. 77–79, 1985. View at Publisher · View at Google Scholar · View at Scopus
  31. T. J. Sill and H. A. von Recum, “Electrospinning: applications in drug delivery and tissue engineering,” Biomaterials, vol. 29, no. 13, pp. 1989–2006, 2008. View at Publisher · View at Google Scholar · View at Scopus
  32. D. M. Correia, C. Ribeiro, J. C. C. Ferreira et al., “Influence of electrospinning parameters on poly(hydroxybutyrate) electrospun membranes fiber size and distribution,” Polymer Engineering & Science, vol. 54, no. 7, pp. 1608–1617, 2014. View at Publisher · View at Google Scholar · View at Scopus
  33. T. Galo Càrdenas, L. Johana Sanzana, and L. H. Inoccentini Mei, “Synthesis and characterization of chitosan-PHB blends,” Boletín de la Sociedad Chilena de Química, vol. 47, no. 4, pp. 529–535, 2002. View at Publisher · View at Google Scholar
  34. A. C. Mottin, E. Ayres, R. L. Oréfice, and J. J. Drummond Câmara, “What changes in poly(3-hydroxybutyrate) (PHB) when processed as electrospun nanofibers or thermo-compression molded film?” Materials Research, vol. 19, no. 1, pp. 57–66, 2016. View at Publisher · View at Google Scholar · View at Scopus
  35. X. Zhu, W. Cui, X. Li, and Y. Jin, “Electrospun fibrous mats with high porosity as potential scaffolds for skin tissue engineering,” Biomacromolecules, vol. 9, no. 7, pp. 1795–1801, 2008. View at Publisher · View at Google Scholar · View at Scopus
  36. H. Rodriguez-Tobias, G. Morales, A. Ledezma et al., “Electrospinning and electrospraying techniques for designing novel antibacterial poly(3-hydroxybutyrate)/zinc oxide nanofibrous composites,” Journal of Materials Science, vol. 51, no. 18, pp. 8593–8609, 2016. View at Publisher · View at Google Scholar · View at Scopus
  37. K. Pietrucha, “Changes in denaturation and rheological properties of collagen–hyaluronic acid scaffolds as a result of temperature dependencies,” International Journal of Biological Macromolecules, vol. 36, no. 5, pp. 299–304, 2005. View at Publisher · View at Google Scholar · View at Scopus
  38. M. Zündel, E. Mazza, and A. E. Ehret, “A 2.5D approach to the mechanics of electrospun fibre mats,” Soft Matter, vol. 13, no. 37, pp. 6407–6421, 2017. View at Publisher · View at Google Scholar · View at Scopus
  39. Z. Chen, B. Wei, X. Mo, C. T. Lim, S. Ramakrishna, and F. Cui, “Mechanical properties of electrospun collagen–chitosan complex single fibers and membrane,” Materials Science and Engineering: C, vol. 29, no. 8, pp. 2428–2435, 2009. View at Publisher · View at Google Scholar · View at Scopus
  40. Y. Yin, D. Pu, and J. Xiong, “Analysis of the comprehensive tensile relationship in electrospun silk fibroin/polycaprolactone nanofiber membranes,” Membranes, vol. 7, no. 4, p. 67, 2017. View at Publisher · View at Google Scholar
  41. S. Wang, A. Ajji, S. Guo, and C. Xiong, “Preparation of microporous polypropylene/titanium dioxide composite membranes with enhanced electrolyte uptake capability via melt extruding and stretching,” Polymer, vol. 9, no. 12, p. 110, 2017. View at Publisher · View at Google Scholar · View at Scopus
  42. A. Bhattacharjee, K. Kumar, A. Arora, and D. S. Katti, “Fabrication and characterization of pluronic modified poly(hydroxybutyrate) fibers for potential wound dressing applications,” Materials Science and Engineering: C, vol. 63, pp. 266–273, 2016. View at Publisher · View at Google Scholar · View at Scopus
  43. C. Edwards and R. Marks, “Evaluation of biomechanical properties of human skin,” Clinics in Dermatology, vol. 13, no. 4, pp. 375–380, 1995. View at Publisher · View at Google Scholar · View at Scopus
  44. J. G. Fernandes, D. M. Correia, G. Botelho et al., “PHB-PEO electrospun fiber membranes containing chlorhexidine for drug delivery applications,” Polymer Testing, vol. 34, pp. 64–71, 2014. View at Publisher · View at Google Scholar · View at Scopus
  45. K. Mukai, Y. Doi, Y. Sema, and K. Tomita, “Substrate specificities in hydrolysis of polyhydroxyalkanoates by microbial esterases,” Biotechnology Letters, vol. 15, no. 6, pp. 601–604, 1993. View at Publisher · View at Google Scholar · View at Scopus
  46. T. Freier, C. Kunze, C. Nischan et al., “In vitro and in vivo degradation studies for development of a biodegradable patch based on poly(3-hydroxybutyrate),” Biomaterials, vol. 23, no. 13, pp. 2649–2657, 2002. View at Publisher · View at Google Scholar · View at Scopus
  47. D. M. Correia, J. Padrao, L. R. Rodrigues, F. Dourado, S. Lanceros-Mendez, and V. Sencadas, “Thermal and hydrolytic degradation of electrospun fish gelatin membranes,” Polymer Testing, vol. 32, no. 5, pp. 995–1000, 2013. View at Publisher · View at Google Scholar · View at Scopus