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Journal of Healthcare Engineering
Volume 2018, Article ID 8504273, 10 pages
Research Article

A Comprehensive Method for Accurate Strain Distribution Measurement of Cell Substrate Subjected to Large Deformation

1College of Materials Science and Engineering, Sichuan University, Chengdu 610065, China
2College of Architecture and Environment, Sichuan University, Chengdu 610065, China

Correspondence should be addressed to Yuanwen Zou; nc.ude.ucs@wyz

Received 26 July 2017; Revised 11 October 2017; Accepted 23 October 2017; Published 8 January 2018

Academic Editor: Wei Yao

Copyright © 2018 Hong He et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Cell mechanical stretching in vitro is a fundamental technique commonly used in cardiovascular mechanobiology research. Accordingly, it is crucial to measure the accurate strain field of cell substrate under different strains. Digital image correlation (DIC) is a widely used measurement technique, which is able to obtain the accurate displacement and strain distribution. However, the traditional DIC algorithm used in digital image correlation engine (DICe) cannot obtain accurate result when utilized in large strain measurement. In this paper, an improved method aiming to acquire accurate strain distribution of substrate in large deformation was proposed, to evaluate the effect and accuracy, based on numerical experiments. The results showed that this method was effective and highly accurate. Then, we carried out uniaxial substrate stretching experiments and applied our method to measure strain distribution of the substrate. The proposed method could obtain accurate strain distribution of substrate film during large stretching, which would allow researchers to adequately describe the response of cells to different strains of substrate.