Research Article

Comparative Study on Automated Cell Nuclei Segmentation Methods for Cytology Pleural Effusion Images

Algorithm 2

: Isodata thresholding method.
(1)Set the initial value of threshold (T)
(2)Segment the images into two regions (R1 and R2) using T with this formation (R1 < T and R2 ≥ T)
(3)Find the mean intensity level (u1) for R1 and (u2) for R2
(4)Find new threshold value (T = (u1 + u2)/2)
(5)Iterate steps 2 to 4 to find the difference in T. Select T when it is smaller than the predefined parameter.