Application of 3D Printing Technology for Design and Manufacturing of Customized Components for a Mechanical Stretching Bioreactor

Table 1

Design requirements.

---

Design requirements

---

Bioreactor system

Ease of assembling, use, and cleaning

Small footprint (max 40 cm × 50 cm × 30 cm to fit in an incubator)

Watertightness (85–95% humidity in an incubator)

Modularity and customization

Reliability of culture processes (weeks in an incubator)

---

Culture chamber components (in contact with the culture medium and/or cells/tissues)

Cytocompatibility

Sterilizability (preferably by an autoclave)

Water/sterility tightness

Ease of assembly/disassembly under laminar flow hood

Ease of cleaning

---

FDM manufacturing

Supported and unsupported walls minimum thickness (0.66 mm)

Maximum overhang inclination (45°)

Minimum diameter of vertical holes (2 mm)

Backlash between connecting parts (0.1 mm)

---