Cloning Method for Stress-Resistant Gene of Conringia planisiliqua under Drought Stress

Table 5

Parameters of PCR reaction system.

Deionized water	19.3 μL
10 × PCR buffer	2.5 μL
DNTP	2.0 μL
P3	0.5 μL
P5	0.5 μL
The template	A single colony
RTaq enzyme	0.2 μL
The total volume	25 μL