[Retracted] RNF12 Promotes Glioblastoma Malignant Proliferation via Destructing RB1 and Regulating MAPK Pathway

Figure 4

RNF12 mediates RB1 degradation and regulates MAPK pathway. (a, b) Scramble siRNA or RNF12 siRNAs were transfected into glioblastoma cells. After 2 days, the transfection is complete. After cycloheximide treatment, cells were collected at various time intervals and submitted to WB. (c) Scramble siRNA or RNF12 siRNAs were transfected into glioblastoma cells. After 2 days, the transfection is complete. Cells were collected and submitted to WB after being treated with MG132 or DMSO. (d) Gene card’s RNF12 function analysis via signal route. (e) Scramble siRNA or RNF12 siRNAs were used to infect LN229 and U343 cells. Two days after transfection, the levels of endogenous MAPK pathway proteins were measured by western blotting. Three separate tests yielded comparable findings, and 1 sample result was displayed. The data are provided as mean standard deviation. .

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