miR-101-3p Contributes to α-Synuclein Aggregation in Neural Cells through the miR-101-3p/SKP1/PLK2 Pathway

Figure 1

Co-overexpression of miR-101-3p and α-synuclein promotes α-Syn aggregation and neurotoxicity. (a) Heatmap of gene expression between substantia nigra from postmortem brains of patients with Parkinson’s disease and health donors. (b) Volcano map of gene expression between substantia nigra from postmortem brains of patients with Parkinson’s disease and health donors. (c) Western blot analysis of SH-SY5Y after transfection of α-synuclein overexpression plasmid. (d) Immunofluorescence of α-synuclein aggregation. (e) Flow cytometry analysis of cell apoptosis. Error bars represent mean ± SD. . n.s., not significant, by the paired two-sided Student’s t-test.

(a)

(b)

(c)

(d)

(e)