Tunicamycin-Induced Endoplasmic Reticulum Stress Promotes Breast Cancer Cell MDA-MB-231 Apoptosis through Inhibiting Wnt/β-Catenin Signaling Pathway

Figure 3

Tunicamycin inhibits the Wnt/β-catenin signaling pathway to affect the viability of MDA-MB-231 cells. (a) Western blotting show the relative level of apoptosis-related proteins, Bax and Bcl-2, upon various treatments of tunicamycin and LiCl. Three independent experiments were performed, and GAPDH was used as the internal control. (b) The apoptosis in MDA-MB-231 cells was measured using acridine orange/ethidium bromide (AO/EB) staining. (c) The expression of c-Myc was measured using western blot. Three independent experiments were performed, and GAPDH was used as the internal control. Values shown (n = 3) are the mean ± SD, or versus the control group; or compared with Tun + Li group. C group of MDA-MB-231 cells was cultured normally with no treatment; Tun groups were delivered with tunicamycin; Li groups were delivered with LiCl; Tun + Li groups were delivered with tunicamycin and LiCl.

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