[Retracted] Clinical and Imaging Study of Repetitive Transcranial Magnetic Stimulation in the Treatment of Morphine Dependence Through mGluR5/TDP43/NR2B Pathway

Table 2

Reverse transcription reaction system.

Composition	Volume (µl)	Final concentration
DNAse treated RNA	4	—
5 × buffer	4	1
dNTPs (10 mM)	1	0.5 mM
Oligo (dT)18 (50 μM)	1	25 pM
Random primer (100 μM)	0.5	25 pM
MMLV reverse transcriptase (200 U/μl)	1	10 U/µl
DEPC H2O	8.5	—
Total volume	20	—