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Journal of Healthcare Engineering
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2021
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Article
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Tab 4
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Research Article
[Retracted] Clinical and Imaging Study of Repetitive Transcranial Magnetic Stimulation in the Treatment of Morphine Dependence Through mGluR5/TDP43/NR2B Pathway
Table 4
Reaction conditions.
NR2B
GAPDH
Experimental steps
Temperature
Time
Number of cycles
Temperature
Time
Number of cycles
Predenaturation
94°C
2 min
35
94°C
2 min
35
Transsexual
94°C
30 sec
94°C
30 sec
Annealing
52°C
30 sec
55°C
30 sec
Extend
72°C
30 sec
72°C
30 sec
Extend
72°C
10 min
72°C
10 min